Product Name: Progeline
Aliased names: ECM-Protect, TFA-Val-Tyr-Val-OH
Amino acid sequence: TFA-Val-Tyr-Val-OH (trifluoroacetyl-valine-tyrosine-valine)
Molecular weight: 475.46 g/mol
Density: 1.299 ± 0.06 g/cm³
pKa 3.32 ± 0.10 (predicted value)
Appearance: White to off-white powder
Solubility: Water-soluble
Purity ≥95%–98% (HPLC)
Storage Conditions: Lyophilized powder should be stored sealed and protected from light at 2–8°C; for long-term storage, it is recommended to maintain below –15°C.
Note: Trifluoroacetyl tripeptide-2 is an active tripeptide derived from elastase inhibitor (Elafin), composed of three amino acids, whose stability and bioavailability are enhanced through trifluoroacetylation modification.The trifluoroacetyl group exhibits strong electronegativity, enhancing the interaction between peptides and skin receptors as well as cellular components, thereby conferring greater stability.
Trifluoroacetyl Tripeptide-2 (Progeline) — The Progerin-Inhibiting Anti-Aging Peptide
A bioactive tripeptide (TFA-Val-Tyr-Val-OH) derived from the elastase inhibitor Elafin, engineered with a trifluoroacetyl group for enhanced stability and bioavailability. It works by suppressing progerin synthesis — a key driver of cellular aging — while inhibiting MMP-1, MMP-3, MMP-9 and elastase activity to protect the extracellular matrix. Clinically proven to reduce wrinkle depth by 20-30%, improve skin firmness by 15-25%, lift the jawline by 10% in 56 days, and boost skin elasticity by 20% in just 28 days. Also stimulates SIRT-1 and SIRT-3 longevity proteins and syndecan production for comprehensive anti-aging benefits. With a molecular weight under 500 Da, it penetrates the epidermis effectively. A premium ingredient trusted by brands including SkinMedica, Estée Lauder, Olay, and Darphin.
Core Efficacy
Inhibition of progerin synthesis – delaying cellular aging (core efficacy)
Progerin is a variant of Lamin A protein and is recognized as a key inducer of cellular aging.With advancing age, progerin accumulates progressively in the body, leading to nuclear defects and DNA damage, which subsequently induce skin laxity, sagging, and wrinkles.Trifluoroacetyl tripeptide-2 can inhibit the synthesis of progerin at its source, protect the integrity of the nuclear lamina membrane, prolong cell lifespan, and restore cellular activity to levels close to those of young cells.
Inhibition of matrix metalloproteinase (MMP) and elastase activity
Trifluoroacetyl tripeptide-2, as a dual inhibitor of MMPs and elastase, significantly inhibits the activity of MMP-1, MMP-3, and MMP-9, thereby reducing the degradation of collagen and elastin.In vitro studies have demonstrated that it inhibits elastase activity by more than 50%, effectively preserving the integrity of the extracellular matrix (ECM).
Promote the synthesis of polyligand proteoglycan (syndecan)
Significantly increased synthesis of polyligand proteoglycan-1 in human keratinocytes.At concentrations of 0.0005 ppm and 0.005 ppm, the stimulatory effects were approximately +19% and +56%, respectively (p <0.05), comparable to those observed with TGF-β (a known inducer of proteoglycan synthesis), thereby facilitating healthy interactions between cells and the surrounding matrix.
Enhance the expression of longevity proteins (sirtuins)
It stimulates the production of longevity-related proteins SIRT-1 and SIRT-3, reduces stem cell aging, aids in restoring mitochondrial function, and delays the aging process at the cellular energy metabolism level.
Promotes collagen synthesis and enhances skin firmness
By upregulating the expression of genes such as COL1A1 and ELN, it promotes the synthesis of collagen and elastin, restores collagen levels to those of young cells, enhances skin elasticity and firmness, and reduces fine lines and sagging.
Clinical efficacy data
Apply a cream containing 0.005% trifluoroacetyl tripeptide-2 twice daily; after 28 days, skin elasticity improves by approximately 20%, and wrinkles are significantly reduced.
Using a cream containing 2% Progeline resulted in a 10% improvement in the sagging chin line and approximately a 20% increase in skin elasticity and thickness after 56 days.
In vitro facial division study: Reduces wrinkle depth by 20%-30% and enhances skin firmness by approximately 15%-25%.
The overall consumer satisfaction score reaches as high as 92%.
Reference proportions for addition to cosmetics
Lyophilized powder (raw material powder)
Drug dosage form | Recommended addition amount (final pure peptide concentration) | explain |
Serum/Ampoule | 0.005%～0.01%（50～100 ppm） | High-concentration essence products |
Face Cream/Lotion | 0.001%～0.005%（10～50 ppm） | Daily Care Products |
Eye Cream/Cervical Cream | 0.001%～0.005%（10～50 ppm） | For anti-aging needs in the periorbital and cervical areas |
Mask (patch/wash-on type) | 0.005%～0.01%（50～100 ppm） | Intensive Care |
Applicable Product Types
Anti-aging/anti-wrinkle essence, face cream, emulsion
Tightening and enhancement products (for face, neck, and mandibular line)
Eye cream, neck cream
Mask (patch-type/moisturizing type)
Anti-aging products
Postpartum and sun exposure care products
Post-repair products for medical art treatments
Formulation Precautions
Administration timing: Administer during the final stage of cosmetic production (low-temperature phase), with temperature not exceeding 40°C.
Solubility: Water-soluble; good compatibility
pH range: The recommended formulation pH should be controlled between 5.0 and 7.0.
Compatibility: Can be formulated with common ingredients such as vitamin C, hyaluronic acid, nicotinamide, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7.
Collaborative Component Recommendation:
Progeline + Matrixyl 3000 (inhibits progerin protein and promotes collagen synthesis, offering dual anti-aging effects)
Progeline + Hyaluronic Acid (anti-aging and firming + deep hydration)
Progeline + Vitamin C (anti-aging repair + antioxidant brightening)
Safety: Confirmed by skin irritation testing to be non-allergenic; with a molecular weight below 500 Da, it readily penetrates the epidermal layer; trifluoroacetyl modification enhances its stability under light and thermal conditions.
Brand Application Case
Trifluoroacetyl tripeptide-2 has been incorporated into high-end skincare products by several internationally renowned brands, including:
Oaly Pure Radiance Crystal-Translucent Skin Base Serum
Estée Lauder New Elastic Firming Eye Cream
Darphin Silver Diamond Firming & Contouring Cream
SkinMedica TNS Advanced+ Essence Solution
Revision Skincare Revox 7 Essence
