Tripeptide-32

1、 Product basic file

Chinese name: Tripeptide-32

English name: Tripeptide-32

INCI Name: TRIPEPTIDE-32

CAS number: 1185583-20-3

Molecular formula: C ₁₂ H ₂₂ N ₄ O ₅

Molecular weight: 302.33 g/mol

Amino acid sequence: H-Ser-Thr-Pro-NH ₂

Amino acid composition: Composed of serine (Ser), threonine (Thr), and proline (Pro), the C-terminus undergoes amidation modification to enhance stability

Appearance: White to off white solid powder

Purity: ≥ 98% (HPLC)

Solubility: soluble in DMSO, slightly soluble in water (≥ 5 mg/mL)

Storage conditions: Powder state sealed and protected from light, frozen at -20 ℃ for 3 years; Store at 4 ℃ for 2 years; After preparing the solution, store it at -80 ℃ for 1 year to avoid repeated freezing and thawing

2、 Core efficacy and mechanism of action

Tripeptide-32 is a synthetic tripeptide whose core design concept stems from in-depth research on the skin's circadian rhythm. The human skin has an independent "biological clock" system - a molecular timer composed of core biological clock genes such as CLOCK and PER1 and their protein products, which regulates the 24-hour rhythm of the skin to resist external damage during the day and initiate self repair at night. Tripeptide-32 activates these key genes to reconstruct and optimize the circadian rhythm of skin repair, achieving a unique effect of "regulating skin self repair at the genetic level".

Its core efficacy and mechanism of action are mainly reflected in the following dimensions:

Activation of skin circadian clock genes and regulation of circadian repair rhythm (core efficacy): The amino acid sequence Ser Thr Pro (STP motif) of tripeptide-32 is derived from microtubule associated protein Tau and has been shown to be involved in the regulation of circadian rhythm. Tripeptide-32 can accurately activate the CLOCK gene and PER1 gene in keratinocytes. During the day, the expression of CLOCK and PER1 genes is active, effectively regulating the physiological rhythm of the body, regulating the cell cycle, and promoting DNA damage repair; However, at night, gene activity decreases and the self repair process of cells slows down. Tripeptide-32 activates these two key genes, helping the skin maintain efficient repair activity even at night, equivalent to "allowing the skin to repair damaged cells at night just like during the day".

Promoting DNA damage repair and enhancing cell vitality: After activating the CLOCK gene, tripeptide-32 triggers the production of a series of enzyme proteins responsible for DNA damage repair, improving cell vitality and lifespan, and enhancing the skin's defense against environmental damage factors such as ultraviolet radiation. The PER1 gene also encodes proteins involved in the day night DNA repair process. Activation of the PER1 gene by tripeptide-32 can significantly improve the survival rate of keratinocytes, maintain the natural barrier function of the skin, and prevent photoaging.

Moisturizing and Water Locking: Tripeptide-32 has good water binding properties (Humectant), which can attract and lock in moisture in the skin, prevent water loss, and maintain the optimal hydration level of the skin. This is crucial for maintaining skin barrier function and improving skin texture.

Protecting collagen, antioxidant and anti glycation: Tripeptide-32 can protect collagen from damage caused by activated carbon groups, with antioxidant and anti glycation effects, helping to maintain skin firmness and elasticity, and reducing the appearance of fine lines and wrinkles.

Clinical validation data (Est é e Lauder Advanced Night Repair series):

After 8 weeks of use: skin dullness decreased by 42%, fine lines around the eyes decreased by 33%, fine lines and wrinkles on the forehead decreased by 26%, skin uniformity increased by 32%, and glossiness increased by 24%

After 2 hours of use: Skin barrier strengthened by 22%

After 3 weeks of use: 90% of users agree that their skin is firmer

After one night of use: 92% of users agree that their skin is moisturized, and 91% agree that their skin is more hydrated

3、 Recommended addition ratio and application scenarios

Tripeptide-32 formula has good compatibility and can be added to a variety of skin care formulations such as essence liquid, face cream, lotion, eye cream, facial mask, etc.

Recommended addition ratio:

Pure peptide powder purity: ≥ 98% (HPLC)

Recommended dosage for solution: 100-1000 ppm (based on pure peptide), to be used in combination with other active ingredients

Commercialized solution composition: Tripeptide-32+Glycerol+Octanol+Ethylhexylglycerin+Water (specific concentration can be customized according to customer needs)

Night Repair essence/face cream: as a core biological clock regulator, it uses its ability to activate CLOCK and PER1 genes to create a "night repair" concept product for skin repair obstacles caused by staying up late, irregular work and rest, jet lag, etc. Est é e Lauder's Advanced Night Repair series uses tripeptide-32 as the core patented ingredient.

Anti aging products: Utilizing its multiple anti-aging mechanisms that promote DNA repair, protect collagen, and enhance cell vitality, applied to anti-aging care products for the face, neck, and hands.

Moisturizing and repairing products: Utilizing their moisturizing and barrier strengthening functions, applied to daily moisturizing and repairing products to help the skin maintain a hydrated barrier state.

Medical art post repair products: utilizing their ability to promote cell repair and regeneration, applied to medical art post skin repair care. Est é e Lauder officially states that its products are "suitable for use after medical beauty treatments".

4、 Formula recommendations and safety compliance

Gold synergy combination:

Combined with hyaluronic acid: Tripeptide-32 regulates the repair rhythm at the genetic level, while hyaluronic acid provides immediate deep moisturizing, achieving a dual effect of "repair+moisturizing" through their synergistic action. The Est é e Lauder Brown Bottle series uses the classic combination of tripeptide-32+hyaluronic acid.

Compound with fermentation products of split yeast: split yeast exerts its anti redness and anti-inflammatory effects by inhibiting inflammatory factors, while tripeptide-32 activates repair programs at the genetic level, forming a synergistic "anti-inflammatory+repair" scheme.

Combined with antioxidant components such as resveratrol and vitamin E, tripeptide-32 activates repair genes, while antioxidant components neutralize free radicals to reduce the source of damage, forming a "defense+repair" synergistic scheme.

Combined with ceramide: Tripeptide-32 promotes repair at the genetic level, while ceramide strengthens the barrier at the lipid level, achieving a more comprehensive barrier repair effect through both internal and external modifications.

Compound with baobab tree seed extract: The combination used in Est é e Lauder's small brown bottle formula synergistically enhances nighttime repair efficacy.

Compatibility and process suggestions: Tripeptide-32 is recommended to be added in the post formulation stage under low temperature conditions (<40 ℃) to maximize its biological activity. It is recommended to refer to the supplier's technical documentation for the pH range.

Safety compliance: Tripeptide-32 is a pure synthetic tripeptide, not of animal origin, with high safety and minimal side effects. It has been included in the cosmetics raw material database and meets the regulatory requirements for cosmetics both domestically and internationally. Est é e Lauder products have been tested by dermatologists and ophthalmologists and are suitable for all skin types, including sensitive skin.

5、 Application Fields and Market Supply

Tripeptide-32, as a representative raw material of "skin circadian clock regulating peptides", has a wide range of applications in the night repair and anti-aging skincare market.

Research and Development Background: Tripeptide-32 (Chronolux) ®） The inspiration for its development comes from the scientific discovery of the circadian rhythm of the skin. Modern lifestyles such as staying up late, exposure to blue light, and jet lag can disrupt the skin's biological clock, leading to decreased repair ability and accelerated aging. Tripeptide-32 accurately activates CLOCK and PER1 genes by simulating the natural STP motif, helping the skin rebuild a healthy circadian rhythm of repair.

Benchmark product application: Tripeptide-32 is the most famous application as the core patent ingredient of Estee Lauder Advanced Night Repair (special moisturizing and repairing essence/small brown bottle). Est é e Lauder claims that after testing 60 million peptides, tripeptide-32 is the only ingredient that can "reset the rhythm of skin repair". This product series has become a benchmark product for night repair and skincare worldwide, fully verifying the market value and technical strength of tripeptide-32.

Market prospect: As consumers' awareness of "scientific skin care" and "precise skin care" continues to improve, and the problem of skin biological clock disorder caused by modern lifestyles is becoming increasingly common, tripeptide-32, as a pioneer raw material in the field of "skin biological clock regulation", has broad application prospects in the sub markets of night repair essence, anti-aging face cream, medical art post repair, etc.